TB-500

10MG per vial

TB-500 is a trade designation applied to synthetic material derived from the actin-binding region of thymosin beta-4, a 43-residue intracellular polypeptide that binds monomeric G-actin in a 1:1 complex and buffers the monomer pool available for polymerisation into F-actin. The name is used inconsistently across the research market: it has been applied both to the N-terminally acetylated heptapeptide Ac-Leu-Lys-Lys-Thr-Glu-Thr-Gln, corresponding to residues 17-23 of thymosin beta-4 and containing the central LKKTET actin-binding motif, and to the full-length 43-residue sequence. Analytical characterisation of a marketed TB-500 preparation by high-resolution mass spectrometry identified the acetylated 17-23 fragment, but the two candidate species are chemically distinct and differ in mass by more than five-fold, so the certificate of analysis for a given lot is what establishes which one is present.

Repair & Regeneration Models

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Research use only. Supplied for in vitro laboratory research. Not a drug, supplement, cosmetic or medical device, not approved by the TGA, and not for administration to humans or animals. See our research use conditions.

Compound signature

From confirmed structure

Characterised targets

G-actin

Research applications

In vitro studies have quantified actin monomer sequestration and inhibition of filament assembly using DNase I inhibition and pyrene-actin polymerisation assays. Structural work has shown that the intact 43-mer binds G-actin in an extended conformation, contacting both the barbed and pointed ends through an N-terminal helix, the central LKKTET segment and a C-terminal segment, so the heptapeptide reproduces only part of that interaction surface; comparative assays have nonetheless reported similar activity for the short motif and the full-length peptide in endothelial migration and vessel-sprouting models, with peptides lacking the motif inactive. Cell-based work has examined directional migration of human umbilical vein endothelial cells and of corneal epithelial cells, the latter alongside laminin-5 expression at the basement membrane, and rodent studies following coronary artery ligation have investigated formation of a complex with PINCH and integrin-linked kinase and the associated Akt signalling in cardiac tissue. Most of this literature was generated with the full-length peptide.

Formulation and handling

Both candidate forms are strongly hydrophilic and enter aqueous solution readily, but they are not equivalent chemically: the full-length 43-mer is markedly acidic with a low isoelectric point, whereas the acetylated heptapeptide is close to net-neutral at physiological pH. The lyophilised cake is light and easily disturbed, so introduce diluent slowly down the vial wall rather than directly onto the powder, and confirm from the certificate of analysis which species the vial holds before calculating molar concentrations.

Intended use

TB-500 is supplied exclusively for in vitro and non-clinical scientific research. It is not approved for human or animal therapeutic, diagnostic or medicinal use. All handling and experimentation must be carried out by trained personnel in an appropriately controlled laboratory.

Specification

Presentation: Vial
Labelled strength: 10MG
Molecular targets: G-actin
Appearance: Lyophilised powder
Storage: 2–8 °C sealed, −20 °C long term

Values describe the compound as supplied. Analytical figures for the material in your vial are on that lot’s certificate, not here.

Certificates of analysis

None published yet

Every lot is tested by an independent laboratory and carries its own certificate. Match the lot number printed on your vial to the certificate — a certificate for a different lot tells you nothing about yours.

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